WebSep 18, 2015 · Transfection of TSC2 into p53 null cells replaced TSC2 and diminished Rheb at the lysosome, recapitulating cells with wt p53. ... They included rabbit anti-TSC2 (CST, 4308, 1:800), ... WebGlaciecola sp. kul10 es una bacteria que se encuentra en ambientes extremos como los glaciares y los océanos. Su capacidad para producir enzimas y compuestos bioactivos la hace de interés para la investigación en campos como la …
IMPDH inhibitors for antitumor therapy in tuberous sclerosis …
WebMay 17, 2024 · Loss-of-function mutations in TSC1 or TSC2, but not TBC1D7, give rise to TSC, a pleiotropic disorder with aberrant ... CST #5425) was used as a blocking solution for 1 h at room temperature. Primary antibodies, p(S235/236)S6 (CST #4858, 1:400), fast skeletal Myosin (Sigma #M4276, 1:400), and slow skeletal Myosin (Abcam # ... WebAug 1, 2024 · AbstractTuberous sclerosis complex (TSC) is caused by mutations of either the TSC1 or TSC2 tumor suppressor gene. TSC causes tumors of the brain, heart, kidney, skin and lymphangioleiomyomatosis (LAM). Here we report that the TSC2 protein physically binds to high-density lipoprotein binding protein (HDLBP), also called vigilin, a core stress … ray whittier
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WebSep 18, 2015 · In cells with basal wt p53 function, p53-dependent transcription supplies adequate levels of TSC2 and sestrin2 to regulate mTORC1 kinase activity; when cells lose p53 transcriptional activity, TSC2 and Sestrin2 levels are low, TSC2 binding at the lysosomal sites of mTORC1 is low, Rheb levels are higher at these sites, and PRAS40 is lower in … WebFor immunoprecipitation of endogenous TSC and mTOR complexes, cell lysates were incubated with TSC2 antibody (CST, 4308) or mTOR antibody (CST, 2972) at 4oC with rocking for 4 hours, followed by an additional 2 hour incubation with 30µl of 50% slurry A/G-agarose beads (Thermo Scientific/Pierce, 20421). WebMonoclonal Antibody for studying Raptor. Cited in 516 publications. Validated for Western Blotting, Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously … simply thick insurance coverage