Cholesterol efflux assay protocol

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August undefined, 2024

WebMar 6, 2012 · Treatment of apoA-I and HDL with MMP-8 resulted in significant reduction (up to 84%, P < 0.001) in their ability to facilitate cholesterol efflux from cholesterol-loaded THP-1 macrophages. The cleavage of apoA-I by MMP-8 and the reduction in its cholesterol efflux capacity was inhibited by doxycycline. WebSummaryAutomatic TranslationMarch 6th, 2012. The cholesterol assay is designed to quantitate the rate of cholesterol efflux from cultured cells and the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of labelling cells with cholesterol, equilibration of cholesterol among intracellular pools and release ...

Cholesterol Uptake Capacity: A New Measure of HDL …

WebThe cholesterol efflux assay is designed to quantitate the rate of cholesterol efflux from cultured cells. It measures the capacity of cells to maintain cholesterol efflux and/or the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of the following steps. WebCholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive, and reproducible method for measuring cholesterol efflux. ... Cholesterol Efflux Assay Protocol: The procedure described below is for macrophage cell line J774.1 ... city of london provincial offences

Cell-free Biochemical Fluorometric Enzymatic Assay for High …

WebMar 22, 2024 · The cholesterol efflux assay showed no difference between HBP treated and untreated with TNFα for 24 h in the presence of HDL and APOA-I ... The resazurin assay was performed based on the protocol of Jennings et al. . A resazurin stock solution at 880 µM (R7017, Sigma) was prepared by dissolving the powder in 0.1 N NaOH (0.011 … WebCholesterol Efflux Assay Protocol The procedure described below is for murine macrophage cell line J774.1. This procedure can also be used with macrophage cells derived from THP-1 monocytes, which can be differentiated into cells with a macrophage-like phenotype upon treatment with 100 nM phorbol 12-myristate-13-acetate (PMA) for … WebMay 21, 2024 · Perhaps the most interesting finding from our study was that palmitoleate was sufficient to induce the activation of Akt in the THP-1 macrophages and the inhibition of apoA-I- and HDL-mediated cholesterol efflux, while oleate at a 10.2-fold higher concentration failed to significantly affect these processes. city of london recreation registration

K582-100 Cholestrol Efflux Assay Kit (Cell-Based) - BioVision

BODIPY-cholesterol can be reliably used to monitor cholesterol …

WebAug 12, 2024 · Cholesterol efflux assay Request a detailed protocol. ... (IACUC) protocols (#07919–2024) of Yale University. At 10 weeks of age, male C57BL/6 J mice (JAX, #000664) were injected with 15 mg/kg lipopolysaccharide (LPS) from E. coli O111:B4 intraperitoneally (Sigma). Six hr later, blood was collected for lipid and cytokine analysis. city of london public transitWebVII. Cholesterol Efflux Assay Protocol: The procedure described below is for murine macrophage cell line J774.1. This procedure can also be used with macrophage cells derived from THP-1 monocytes, which can be differentiated into cells with a macrophage-like phenotype upon treatment with 100 nM phorbol 12-myristate-13 … city of london public pools

"WebCholesterol Assay Kit (Cell-Based) (ab133116) includes filipin III, fixative, and wash buffer in a ready to use format. It provides a simple fluorometric method to study mechanisms and biological factors that regulate cholesterol metabolism or movement within cells. A cholesterol trafficking inhibitor, U-18666A, is included as a positive control. " - Cholesterol efflux assay protocol

Cholesterol efflux assay protocol

A sensitive assay for ABCA1-mediated cholesterol efflux using …

WebNational Center for Biotechnology Information WebThe cellular cholesterol efflux assay is designed to quantify the rate of cholesterol efflux from cultured cells to an acceptor particle or to plasma. This assay is multi step, cell based assay. Various factors, if not carefully controlled may influence the accuracy and reproducibility of the assay.

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WebThe Cholesterol Efflux Assay Kit is a high-throughput screening assay that measures cholesterol efflux in cells using fluorescent-labeled cholesterol (λ Ex = 482 nm/ λ Em = 515 nm). It is suitable for serum, isolated, or recombinant lipoprotein samples. WebOct 12, 2024 · Here a protocol is describedfor determination of HDL-lipid peroxidation using the fluorochrome reagent. Assay variability can be reduced by strict standardization of experimental conditions. ... This approach avoids known limitations of prior HDL function assays (e.g. the cholesterol efflux assay) including significant heterogeneity with ...

WebThe cholesterol efflux assay protocol uses fluorescently-labeled cholesterol (Ex/Em 482/515 nm). It provides a safe, sensitive and reproducible method for measuring cholesterol efflux. - screen small molecules for their effect on cholesterol efflux as a part … WebCholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive, and reproducible method for measuring cholesterol efflux.

WebAug 13, 2024 · In some experiment, cellular cholesterol was trace-labeled using protocol described for cholesterol efflux, and the radioactivity in fractions were analyzed on a liquid scintillation counter. Lipidomics. ... WebFluorescent-labelled cholesterol is a promising strategy to analyze and investigate the properties and metabolism of natural cholesterol in vitro. Its main advantages are that it can be taken up by cells, allows for intracellular and membrane distribution studies, and can be applied to cholesterol efflux assays such as in this protocol (Figure 7).

WebAssay Cholesterol Uptake in cell samples with Cholesterol Uptake Assay Kit ab236212. Abcam offers > 1,000 assay kits cited in > 3,500 publications. ... Cholesterol Efflux Assay Kit (Cell-based) (ab196985) ... Please refer to protocols. Components 1 kit; Cell-Based Assay Buffer Tablet: 1 tablet: Cell-Based Assay NBD Cholesterol:

WebDec 1, 2011 · The present study was designed to determine whether a cholesterol efflux assay could be developed that avoided the need to use donor cells that contained radiolabeled cholesterol and eliminated some of the steps needed to establish the efflux capacity of serum, HDL, or apolipoproteins. city of london pubWebMar 17, 2024 · The following cholesterol efflux assay is adapted from a previous protocol performed on macrophages, with modifications. 2 The protocol below describes a detailed step-by-step procedure for preparing the key reagent for this assay, BODIPY-cholesterol, as well as the specific steps for using primary cortical astrocytes from apolipoprotein E … city of london recruitmentWebMar 6, 2012 · Cholesterol efflux assay is usually used to assess the influence of natural products on plasma acceptorinduced cholesterol efflux from cells (Low et al., 2012). Dr. Rothblat's group pioneered ... city of london registrationWebJan 5, 2024 · The following cholesterol efflux assay is adapted from a previous protocol performed on macrophages, with modifications.2 The protocol below describes a detailed step-by-step procedure for preparing the key reagent for this assay, BODIPY-cholesterol, as well as the specific steps for using primary cortical astrocytes from apolipoprotein E ... city of london recreationWebMar 6, 2012 · The cholesterol efflux assay is designed to quantitate the rate of cholesterol efflux from cultured cells. It measures the capacity of cells to maintain cholesterol efflux and/or the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of the following steps. doon international school baramullaWebK603-100 is the same size as the 100 test size of ab65359. Other cholesterol assay kits include: - HDL and LDL/VLDL Cholesterol assay kit ab65390. - C ell-based Cholesterol assay kit ab133116. - Cholesterol … doon house cashelWebAug 9, 2016 · Radioactive in vitro cholesterol efflux assay (CEA) is the gold standard for determination of efflux at cellular level. Fluorescent tracers and stable isotope-labeled cholesterol... city of london red badge