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WebApplied Biosystems SYBR Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and SYBR Green dye—everything needed for real-time PCR. ... Bio-Rad iQ™5 and CFX™ (96 and 384), and Roche ... Web172-5102 ™iTaq Fast SYBR® Green Supermix With ROX, 1,000 x 20 µl reactions 172-5103 ®iTaq™ Fast SYBR Green Supermix With ROX, 20 ml bottle, 2,000 x 20 µl reactions 172-5105 iTaq Fast Supermix With ROX, 200 x 20 µl reactions, 2x mix contains dNTPs, iTaq DNA polymerase, 6 mM Mg2+, ROX passive reference dye, stabilizers citizens advice county durham facebook
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WebThe cDNA template from each sample was mixed with iTaq™ Universal SYBR® Green Supermix (Bio-Rad, Hercules, CA, USA) and gene-specific mouse primers (Table 1). RT-qPCR was carried out with an ABI 7300 Real-time PCR system (Life Technologies, Grand Island, NY, USA) to obtain CT values. WebMar 3, 2024 · Quantitative PCR was performed in 20 μL reactions with 200 nM of each primer, iTaq Universal SYBR Green Supermix (Biorad, Bio-Rad, Des Plaines, IL, USA) and 1 μL of cDNA template using the QuantStudio 3 Real-time PCR system (Applied Biosystems, Waltham, MA, USA). Primers used were: GNMT forward 5′ … WebFeb 24, 2012 · Introducing iTaq Universal Supermixes for Highest Quality qPCR Results on Any Instrument. Bioradiations Staff — February 24, 2012. 1.9k 0. The iTaq™ universal SYBR® Green supermix and iTaq™ universal probes supermix are ready-to-use 2x supermixes formulated for sensitive, efficient, and reproducible qPCR data on any … dick butkus autograph signing